The default settings respect the OPC DA 3.0 specification. This means that the checkbox is enabled. Changing the settings make them non-compliant, and enables the other checkboxes. But if none of the other checkboxes are changed, the project is still compliant and the checkbox will be enabled the next time that the Project Properties are loaded. If one of the other checkboxes changes, the "Perform the following operations in agreement with the OPC-DA 3.0 specification" checkbox will not be reset.